Development of a loop-mediated isothermal amplification assay for visual detection of Mycoplasma iowae

A loop-mediated isothermal amplification(LAMP) assay was developed for detection of Mycoplasma iowae(MI).According to the sequences specific to species protein1 of MI in GenBank,six primer pairs were designed,and the reaction conditions were optimized. In result,only LAMP reactions of Mycoplasma iowae were positive(visible green),while LAMP reactions of the other common pathogens were negative(visible red).The detection limit of this LAMP method was 10 fg for DNA,which was 100 times higher than routine PCR.The amplification could be finished within 1 h,and the presence of MI could be detected by naked eyes.These results show that this LAMP assay is a simple and specific method for rapid detection of MI for clinical samples.