多铜氧化酶
白色念珠菌
铁质
还原酶
生物
酵母
铁
生物化学
酿酒酵母
菌丝
微生物学
真菌蛋白
酶
化学
漆酶
有机化学
作者
Rose E. Jeeves,Robert P. Mason,Alexandra Woodacre,Annette Cashmore
出处
期刊:Yeast
[Wiley]
日期:2011-08-08
卷期号:28 (9): 629-644
被引量:24
摘要
Abstract The pathogenic yeast Candida albicans possesses a reductive iron uptake system which is active in iron‐restricted conditions. The sequestration of iron by this mechanism initially requires the reduction of free iron to the soluble ferrous form, which is catalysed by ferric reductase proteins. Reduced iron is then taken up into the cell by a complex of a multicopper oxidase protein and an iron transport protein. Multicopper oxidase proteins require copper to function and so reductive iron and copper uptake are inextricably linked. It has previously been established that Fre10 is the major cell surface ferric reductase in C. albicans and that transcription of FRE10 is regulated in response to iron levels. We demonstrate here that Fre10 is also a cupric reductase and that Fre7 also makes a significant contribution to cell surface ferric and cupric reductase activity. It is also shown, for the first time, that transcription of FRE10 and FRE7 is lower in hyphae compared to yeast and that this leads to a corresponding decrease in cell surface ferric, but not cupric, reductase activity. This demonstrates that the regulation of two virulence determinants, the reductive iron uptake system and the morphological form of C. albicans , are linked. Copyright © 2011 John Wiley & Sons, Ltd.
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