光热治疗
内吞作用
显微镜
氧化铁纳米粒子
荧光显微镜
纳米技术
生物物理学
人口
材料科学
纳米颗粒
右旋糖酐
荧光
细胞
化学
生物
光学
生物化学
社会学
人口学
物理
作者
Lara K. Bogart,Arthur Taylor,Yann Cesbron,Patricia Murray,Raphaël Lévy
出处
期刊:ACS Nano
[American Chemical Society]
日期:2012-06-07
卷期号:6 (7): 5961-5971
被引量:49
摘要
A detailed understanding of cellular interactions with superparamagnetic iron oxide nanoparticles (SPIONs) is critical when their biomedical applications are considered. We demonstrate how photothermal microscopy can be used to follow the cellular uptake of SPIONs by direct imaging of the iron oxide core. This offers two important advantages when compared with current strategies employed to image magnetic cores: first, it is nondestructive and is therefore suitable for studies of live cells and, second, it offers a higher sensitivity and resolution, thus allowing for the identification of low levels of SPIONs within a precise subcellular location. We have shown that this technique may be applied to the imaging of both cell monolayers and cryosections. In the former we have demonstrated the role of temperature on the rate of endocytosis, while in the latter we have been able to identify cells labeled with SPIONs from a mixed population containing predominantly unlabeled cells. Direct imaging of the SPION core is of particular relevance for research involving clinically approved SPIONs, which do not contain fluorescent tags and therefore cannot be detected via fluorescence microscopy.
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