ABSTRACT Selected conditions were examined for their possible roles in the regulation of catalase activity in Staphylococcus aureus MF‐31. The addition of the heme precursor 5‐aminolevulinic acid resulted in increased catalase activity whereas the addition of exogenous hydrogen peroxide did not. Catalase activity decreased when S. aureus MF‐31 cells were grown in media containing glucose. Cells grown in media with succinate or citrate substituted for glucose exhibited increased catalase activity. Cells grown in tryptic soy broth (0.5% NaCl) supplemented with NaCl showed increased catalase activity at salt concentrations up to 3%.