角鲨烯
大肠杆菌
ATP合酶
突变体
生物化学
甲戊酸途径
角鲨烯单加氧酶
化学
生物
生物合成
酶
基因
作者
Akinori Katabami,Ling Li,Miki Iwasaki,Maiko Furubayashi,Kyoichi Saito,Daisuke Umeno
标识
DOI:10.1016/j.jbiosc.2014.07.013
摘要
Squalene is a precursor of thousands of bioactive triterpenoids and also has industrial value as a lubricant, health-promoting agent, and/or drop-in biofuel. To establish an efficient Escherichia coli-based system for squalene production, we tested two different squalene synthases and their mutants in combination with precursor pathways. By co-expressing a chimeric mevalonate pathway with human or Thermosynechococcus squalene synthase, E. coli accumulated squalene up to 230 mg/L or 55 mg/g-DCW in flask culture. We also determined that a significant truncation of squalene synthase at the C-terminus retains partial cellular activity. The squalene-producing strain described herein represents a convenient platform for gene discovery and the construction of the pathway toward natural and non-natural hopanoids/steroids.
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