生物降解
蓝藻
化学
重组DNA
微囊藻毒素
拉伤
细菌
降级(电信)
细菌菌株
基因
色谱法
生物
生物化学
遗传学
有机化学
解剖
电信
计算机科学
作者
Huasheng Wang,Hai Yan,Xiao Lu Liu,Chun Hua Yin,Le Lv,Qian Xu
出处
期刊:Advanced Materials Research
日期:2013-01-01
卷期号:647: 338-343
被引量:2
标识
DOI:10.4028/www.scientific.net/amr.647.338
摘要
Harmful cyanobacterial blooms are a growing environmental problem worldwide in natural waters, and the biodegradation is found to be the most efficient method for removing microcystins (MCs) produced by harmful cyanobacteria. Based on the isolation of a promising bacterial strain of Sphingopyxis sp. USTB-05 for biodegrading MCs, a gene USTB-05-A (HM245411) that is responsible for the first step in the biodegradation of MCs was cloned and expressed for the first time. The cell-free extracts (CE) of recombinant E. coli DH5α containing USTB-05-A had high activity for biodegrading MCs. Based on an analysis of the liquid chromatogram-mass spectrum (LC-MS), the enzyme encoded by gene USTB-05-A was found to be active in cleaving the target peptide bond between Adda and arginine, and converting cyclic MC-RR and MC-YR) to linear MC-RR and MC-YR as their first degradation products, respectively, which offered direct evidence for the first step on the pathway of MCs biodegradation by Sphingopyxis sp. USTB-05.
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