Ultrastructural Properties of Interstitial Cells of Cajal in the Guinea Pig Bladder

PURPOSE: We investigated the ultrastructural characteristics of interstitial cells of Cajal in the guinea pig bladder. MATERIALS AND METHODS: Bladders were removed from guinea pigs and processed for transmission electron microscopy. Some sections were labeled with c-Kit antibodies and colloidal gold particles for positive identification of interstitial cells of Cajal. RESULTS: Kit positive cells, identified with 10 nm gold particles, were located on the periphery of detrusor smooth muscle bundles and in the interbundle spaces. Interstitial cells of Cajal in these regions contained mitochondria, rough and smooth endoplasmic reticulum, thin and intermediate filaments, caveolae, Golgi apparatus, free ribosomes, cytoplasmic vesicles and had a discontinuous basal lamina. They were distinct from smooth muscle cells by an absence of dense bodies, membrane attachment bands and thick filaments. The ultrastructure of interstitial cells of Cajal in all regions of the bladder wall examined were similar and the myofibroblast characteristic, fibronexus, was not evident in any of the cells examined. Interstitial cells of Cajal had lateral branches which extended toward other interstitial cells of Cajal, neighboring smooth muscle cells or nerves. Cells with the ultrastructural profile of interstitial cells of Cajal were associated with bladder microvessels and their branched processes were in close proximity to vascular smooth muscle cells. CONCLUSIONS: Bladder interstitial cells of Cajal have typical ultrastructural characteristics of interstitial cells of Cajal from other tissues and were immunopositive for the interstitial cell of Cajal marker Kit. They were closely associated with detrusor smooth muscle and often networked with other interstitial cells of Cajal. The observation of perivascular cells with interstitial cells of Cajal characteristics indicates that there may be more subtypes of these cells in the bladder than previously considered.