Evaluation of Photobiogoverning Role of Blue Light Irradiation on Viral Replication

Abstract Most recently, severe acute respiratory syndrome coronavirus‐2 has triggered a global pandemic without successful therapeutics. The goal of the present study was to define the antiviral effect and therapeutic action of blue light irradiation in SARS‐CoV‐2‐infected cells. Vero cells were infected with SARS‐CoV‐2 (NCCP43326) or mock inoculum at 50 pfu/well. After blue light irradiation, the inhibitory effect was assessed by qPCR and plaque reduction assay. When Vero cells were irradiated to blue light ranging from 1.6 to 10 J cm −2 , SARS‐CoV‐2 replication was inhibited by up to 80%. The antiviral effect of blue light irradiation was associated with translation suppression via the phosphorylation of eIF2α by prolonging endoplasmic reticulum (ER) stress. The levels of LC3A/B and Beclin‐1, which are key markers of autophagy, and the levels of PERK and PDI for ER stress were highly increased, whereas caspase‐3 cleavage was inhibited after blue light irradiation in the later stage of infection. Our data revealed that blue light irradiation exerted antiviral and photo‐biogoverning activities by prolonging ER stress and stimulating autophagy progression during viral infection. The findings increase our understanding of how photo‐energy acts on viral progression and have implications for use in therapeutic strategies against COVID‐19.