脱氧核酶
化学
丁醇
生物传感器
水溶液中的金属离子
DNA
生物分子
正丁醇
金属
组合化学
纳米技术
无机化学
作者
Yuqing Li,Mohamad Zandieh,Juewen Liu
标识
DOI:10.1002/asia.202101091
摘要
Understanding the activity of biomolecules in cosolvent systems is important for catalysis, separation and developing biosensors. The majority of previously studied solvents are either phase separated with water or miscible with water. Butanol was recently used to extract water for the conjugation of DNA to gold nanoparticles. In this work, the effect of butanol on the activity of a few RNA-cleaving DNAzymes was studied. A 130-fold improvement in sensitivity for the Na+ -specific EtNa DNAzyme was observed, and butanol also improved the activity of another Na+ -specific DNAzyme, NaA43T by a few folds. However, when divalent metal ions were used for both EtNa and 17E DNAzymes, the activity was inhibited. A main driven force for enhanced DNAzyme activity is the concentration effect due to butanol dehydration. This study provides insights into the interplay between DNA, metal ions and organic solvents, and such an understanding might be useful for developing sensitive biosensors.
科研通智能强力驱动
Strongly Powered by AbleSci AI