Homogeneous Biocatalysis in both Fluorous Biphasic and Supercritical Carbon Dioxide Systems

HIP to be green: Cytochrome c and α-chymotrypsin (CMT) can be solubilized in either fluorous solvents or supercritical CO2 by hydrophobic ion pairing (HIP) with perfluorinated anionic surfactants (Krytox 157 FSL, KDP 4606). A model system for homogeneous fluorous biphasic biocatalysis involving the CMT–KDP complex in hexane/perfluoromethylcyclohexane is reported that simplifies both product separation and recycling of the biocatalyst. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2007/z701488_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.