Endothelium‐Mimicking Surface Combats Thrombosis and Biofouling via Synergistic Long‐ and Short‐Distance Defense Strategy

Abstract Thrombosis and infections are the main causes of implant failures (e.g., extracorporeal circuits and indwelling medical devices), which induce significant morbidity and mortality. In this work, an endothelium‐mimicking surface is engineered, which combines the nitric oxide (NO)‐generating property and anti‐fouling function of a healthy endothelium. The released gas signal molecules NO and the glycocalyx matrix macromolecules hyaluronic acid (HA) jointly combine long‐ and short‐distance defense actions against thrombogenicity and biofouling. The biomimetic surface is efficiently fabricated by cografting a NO‐generating species (i.e., Tri‐tert‐butyl 1,4,7,10‐Tetraazacyclododecane‐1,4,7,10‐tetraacetate‐chelated Cu2+, DTris@Cu) and the macromolecular HA on an aminated tube surface through one‐pot amide condensation chemistry. The active attack (i.e., NO release) and zone defense (i.e., HA tethering) system endow the tubing surface with significant inhibition of platelets, fibrinogen, and bacteria adhesion, finally leading to long‐term anti‐thrombogenic and anti‐fouling properties over 1 month. It is envisioned that this endothelium‐mimicking surface engineering strategy will provide a promising solution to address the clinical issues of long‐term blood‐contacting devices associated with thrombosis and infection.