再生(生物学)
骨骼肌
心肌细胞
病变
医学
肌肉组织
体内
病理
解剖
生物
生物医学工程
细胞生物学
生物技术
作者
Yori Endo,Mehran Karvar,Indranil Sinha
出处
期刊:Bio-protocol
[Bio-Protocol]
日期:2021-01-01
卷期号:11 (11)
被引量:4
标识
DOI:10.21769/bioprotoc.4036
摘要
Cryoinjury, or injury due to freezing, is a method of creating reproducible, local injuries in skeletal muscle. This method allows studying the regenerative response following muscle injuries in vivo, thus enabling the evaluation of local and systemic factors that influence the processes of myofiber regeneration. Cryoinjuries are applicable to the study of various modalities of muscle injury, particularly non-traumatic and traumatic injuries, without a loss of substantial volume of muscle mass. Cryoinjury requires only simple instruments and has the advantage over other methods that the extent of the lesion can be easily adjusted and standardized according to the duration of contact with the freezing instrument. The regenerative response can be evaluated histologically by the average maturity of regenerating myofibers as indicated by the cross-sectional areas of myofibers with centrally located nuclei. Accordingly, cryoinjury is regarded as one of the most reliable and easily accessible methods for simulating muscle injuries in studies of muscle regeneration.
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