Expression of miR-200a in colorectal carcinoma cell lines and its effect on LoVo cells

Objective To detect mi R- 200 a expression in human colorectal carcinnoma(CRC) cell lines and explore the role of mi R-200 a in regulating the biological behavior of CRC cells. Methods Real-time quantitative RT-PCR(q RT-PCR) was used to detect mi R- 200 a expression levels in 6 CRC cell lines(HCT116, HT29, LS174 T, SW480, SW620 and Lo Vo). mi R- 200 a mimics were transiently transfected into Lo Vo, and the changes in cell proliferation, apoptosis, migration, and cell-cell adhesion were assessed using CCK- 8 assay, TUNEL assay, transwell migration assay, and homogenous adhesion experiment, respectively.Results The expression of mi R-200 a was down-regulated in the 6 CRC cell lines, among which the highly metastatic Lo Vo cell line showed the lowest expression and the tumorigenic but non-metastatic CRC cell line HCT116 had the highest expression.Overexpression of mi R- 200 a depressed cell proliferation and migration but promoted cell apoptosis and cell- cell adhesion in Lo Vo cells. Conclusion mi R-200 a plays a role in regulating the invasiveness and metastasis of CRC, and overexpression of mi R-200 a causes a significant reduction of cell proliferation and migration and promotes apoptosis and cell-cell adhesion in Lo Vo cells.