FOXO3公司
基因敲除
癌症研究
下调和上调
肝细胞癌
巴西金
竞争性内源性RNA
长非编码RNA
细胞凋亡
生物
转移
转录因子
医学
基因
内科学
癌症
遗传学
基质金属蛋白酶
作者
Lin Yang,Wan-Li Deng,Bao-Guo Zhao,Yao-Zhong Xu,Xiaowen Wang,Fang Yu,Haijuan Xiao
标识
DOI:10.1038/s41417-021-00312-w
摘要
Long non-coding RNAs (LncRNAs) have played very important roles in the malignancy behaviors of hepatocellular carcinoma (HCC). LncRNA LOC554202 (LOC554202) was a newly identified tumor-related lncRNA. However, its expression and function in HCC remained unknown. In this study, we firstly reported that LOC554202 expression was distinctly upregulated in HCC specimens and cell lines. Clinical assays indicated that increased LOC554202 expression had a diagnostic value for HCC patients and was positively associated with advanced stages and poor clinical prognosis. Additionally, forkhead box O3(FOXO3) could bind directly to the LOC554202 promoter region and activate its transcription. Functionally, we observed that knockdown of LOC554202 suppressed the proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT) progress of HCC cells, and promoted apoptosis. Mechanistically, LOC554202 competitively bound to miR-485-5p and prevented the suppressive effects of miR-485-5p on its target gene basigin (BSG), which finally led to HCC metastasis, EMT, and docetaxel chemoresistance. Our data demonstrated that FOXO3-induced LOC554202 contributed to HCC progression by upregulating BSG via competitively binding to miR-485-5p, which suggested that the regulation of the FOXO3/LOC554202/miR-485-5p/BSG axis may have beneficial effects in the treatment of HCC.
科研通智能强力驱动
Strongly Powered by AbleSci AI