小岛
移植
医学
肾包膜
胰腺
胰岛
1型糖尿病
糖尿病
肾
胰岛素
免疫学
内科学
内分泌学
作者
Tatiana Jofra,Giuseppe Galvani,Georgia Fousteri,Silvia Gregori,Nicola Gagliani,Manuela Battaglia
出处
期刊:Bio-protocol
[Bio-Protocol]
日期:2018-01-01
卷期号:8 (5)
被引量:3
标识
DOI:10.21769/bioprotoc.2743
摘要
Type 1 diabetes (T1D) is an autoimmune disease caused by the lack of insulin-producing pancreatic beta cells leading to systemic hyperglycemia. Pancreatic islet transplantation is a valid therapeutic approach to restore insulin loss and to promote adequate glycemic control. Pancreatic islet transplantation in mice is an optimal preclinical model to identify new therapeutic strategies aiming at preventing rejection and optimizing post-transplant immuno-suppressive/-tolerogenic therapies. Islet transplantation in preclinical animal models can be performed in different sites such the kidney capsule, spleen, bone marrow and pancreas. This protocol describes murine islet transplantation under the kidney capsule. This is a widely accepted procedure for research purposes. Stress caused in the animals is minimal and it leads to reliable and reproducible results.
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