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A small molecule, C24H17ClN4O2S, inhibits the function of the type III secretion system in Salmonella Typhimurium

效应器 三型分泌系统 肠沙门氏菌 分泌物 毒力 沙门氏菌 微生物学 生物 致病岛 耶尔森尼亚 肠杆菌科 细菌 细胞生物学 大肠杆菌 生物化学 基因 遗传学
作者
Rerngwit Boonyom,Sittiruk Roytrakul,Patipat Thinwang
出处
期刊:Journal of Genetic Engineering and Biotechnology [Elsevier BV]
卷期号:20 (1): 54-54 被引量:5
标识
DOI:10.1186/s43141-022-00336-1
摘要

Salmonella enterica serovar Typhimurium ( S . Typhimurium) causes gastroenteritis and diarrhea in humans and food-producing animals. The type III secretion system (T3SS) has been known to be a potent virulence mechanism by injecting effector proteins into the cytosol of host cells. S . Typhimurium encodes two T3SSs by Salmonella pathogenicity islands 1 and 2. Previous studies showed that T3SS shared a potent virulence mechanism and molecular structure among several gram-negative bacteria. Therefore, T3SS has been identified as an attractive target in the development of novel therapeutics for the treatment of bacterial infections. Several studies reported that small-molecule compounds are able to inhibit functions of bacterial T3SSs. A small molecule, C 24 H 17 ClN 4 O 2 S, has been shown the ability to inhibit the activity of Yersinia pestis T3SS ATPase, YscN, resulting to block the secretion of effector proteins. In this study, we studied the effects and mechanism for SPI-1 T3SS inhibition of this compound in S. Typhimurium. We demonstrated that this compound prohibited the secretion of effector proteins from Salmonella via SPI-1 T3SS at 100 μM. As the result, bacterial invasion ability into epithelial cell cultures was reduced. In contrast with previous study, the C 24 H 17 ClN 4 O 2 S molecule did not inactivate the activity of SPI-1 T3SS ATPase, InvC, in Salmonella . However, we studied the global cellular effects of S . Typhimurium after being treated with this compound using a quantitative proteomic technique. These proteomic results showed that the main SPI-1 transcription regulator, InvF, and two effector proteins, SipA and SipC, were reduced in bacterial cells treated with the compound. It may explain that action of the small-molecule compound, C 24 H 17 ClN 4 O 2 S, for blocking the secretion of SPI-1 T3SS in Salmonella is through inhibition of SPI-1 regulator, InvF, expression. Further studies are necessary to identify specific mechanisms for inhibition between this small-compound and InvF SPI-1 regulator protein.
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