漆酶
阿布茨
化学
食品科学
地衣芽孢杆菌
阿魏酸
云芝
生物化学
枯草芽孢杆菌
酶
抗氧化剂
生物
细菌
DPPH
遗传学
作者
Yongpeng Guo,Yanan Wang,Yanrong Liu,Qiugang Ma,Cheng Ji,Lihong Zhao
标识
DOI:10.1016/j.lwt.2022.113548
摘要
Zearalenone (ZEN) is an estrogen-like mycotoxin posing a great threat to human and animal health. The present study showed that the induction of spore CotA laccase production of Bacillus licheniformis by ions Mn2+ and Cu2+ promoted the ZEN-degrading ability of the strain. The spore CotA laccase heterologously expressed in Escherichia coli was capable of directly oxidizing ZEN with Km, Kcat and Vmax values of 90.43 μg mL−1, 0.11s−1 and 35.48 μg min−1 mg−1, respectively. The use of 2, 2′-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), acetosyringone, ferulic acid, caffeic acid and methyl syringate as redox mediators could enhance ZEN degradation by CotA laccase. The human breast cancer MCF-7 cell proliferation assay showed that CotA laccase and CotA laccase-mediator systems eliminated the estrogenicity of ZEN. Immobilized CotA laccase onto chitosan microspheres exhibited higher thermal stability over free CotA laccase, maintaining about 87% of its initial activity after heat treatment at 80 °C for 30 min. The removal rate of ZEN in corn meal by free and immobilized CotA laccase was 70% and 90%, respectively. Meanwhile, immobilized enzyme could achieve a 54% ZEN degradation in corn meal after three consecutive cycles. Thus, CotA laccase could be a promising biocatalyst for detoxification of ZEN in corn meal.
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