GRNUlar: A Deep Learning Framework for Recovering Single-Cell Gene Regulatory Networks

We propose GRNUlar, a novel deep learning framework for supervised learning of gene regulatory networks (GRNs) from single-cell RNA-Sequencing (scRNA-Seq) data. Our framework incorporates two intertwined models. First, we leverage the expressive ability of neural networks to capture complex dependencies between transcription factors and the corresponding genes they regulate, by developing a multitask learning framework. Second, to capture sparsity of GRNs observed in the real world, we design an unrolled algorithm technique for our framework. Our deep architecture requires supervision for training, for which we repurpose existing synthetic data simulators that generate scRNA-Seq data guided by an underlying GRN. Experimental results demonstrate that GRNUlar outperforms state-of-the-art methods on both synthetic and real data sets. Our study also demonstrates the novel and successful use of expression data simulators for supervised learning of GRN inference.