Cell imaging with squaraine dye based on two-photon excitation fluorescence imaging

Two-photon excitation fluorescence imaging (TPEFI) is widely used in biomedicine because it has advantages of low cytotoxicity, high resolution and deep imaging depth. In this experiment, OVCAR-3 ovarian cancer cells were labelled with the squaraine dye and detected by confocal laser scanning microscope ( Leica TCS SP8 )for single-photon and two-photon imaging. The results showed that the squaraine dye can be used well to two-photon imaging.