HPLC based estimation and extraction of rutin, quercetin and gallic acid in Moringa oleifera plants grown in Saudi Arabia

Moringa oleifera L. (Moringaceae) is a valuable medicinal plant with a high nutritional and industrial value. To analyse the major polyphenolic compounds rutin, quercetin and gallic acid present in the M. oleifera l eaves, HPLC methods have been used. In the range of 10-30 µg for rutin, quercetin and gallic acid with determinati on coefficient (R2 ) good linearity was observed and linear regression y = 1232.9x + 106.05, y = 133.53x + 202 and y = 61 9.4x + 3442.5 were achieved in 0.98, 0.99 and 1.04 min with the retention time respectively. The LOQs for rutin, quercetin and gallic acid; 3.4, 10.6 and 4.4 µg/ml and LODs ?4.0 µg/ml for all standards were achieved. The rutin (555.6±1.79 µg/g), quercetin (37.54±1.07µg/g) and g allic acid (48.5±1.34 µg/g) were recorded in M. ole fera. Rutin was the most abundant flavonoid analysed in M. oleifera