桡足类
生物
多不饱和脂肪酸
六烯酸
二十碳五烯酸
酵母
脂肪酸
生态学
植物
食品科学
动物科学
生物化学
甲壳动物
作者
Bolette Lykke Holm Nielsen,Hans van Someren Gréve,Thomas Allan Rayner,Benni Winding Hansen
摘要
The cyclopoid Apocyclops royi (Lindberg 1940) and the calanoid Pseudodiaptomus annandalei (Sewell 1919) are 2 tropical copepods suspected of having the capability to biosynthesize the physiologically important n-3 polyunsaturated fatty acids (n-3 PUFAs) eicosapentaenoic acid (C20:5n-3, EPA) and docosahexaenoic acid (C22:6n-3, DHA). We demonstrated this suspected ability using 13 C 18 α-linolenic acid (C18:3n-3, ALA) fed to the copepods through liposomes and a subsequent fatty acid (FA) analysis by GC-MS at 3 different time points (0, 24, and 48 h). Two different diets were applied post liposome exposure: baker’s yeast Saccharomyces cerevisiae and the microalga Dunaliella tertiolecta. For both copepods, further elongated and desaturated 13 C n-3 PUFAs were found at all time points. At T 48h , A. royi and P. annandalei contained 13 C-labelled DHA contents (±SD) of 1.3 ± 0.2 and 0.7 ± 0.3 µg 13 C-FA mgC copepod -1 when fed baker’s yeast, respectively, and 1.2 ± 0.1 and 1.6 ± 0.5 µg 13 C-FA mgC copepod -1 when fed D. tertiolecta , respectively, with significant differences observed only between P. annandalei diet treatments. The 13 C-labelled EPA content of A. royi and P. annandalei at T 48h was 0.6 ± 0.4 and 0.7 ± 0.4 µg 13 C-FA mgC copepod -1 when fed baker’s yeast and 0.8 ± 0.2 and 0.3 ± 0.1 µg 13 C-FA mgC copepod -1 when fed D. tertiolecta , with significant differences only between copepods fed D. tertiolecta . A. royi and P. annandalei exhibited an ability to produce n-3 PUFAs from the precursor ALA in comparatively large quantities. This ability enables these 2 species to inhabit habitats characterized by PUFA-poor particulate material.
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