斑马鱼
达尼奥
杀虫剂
多效唑
氧化应激
化学
毒性
污染物
生物
毒理
药理学
基因
生物化学
生态学
植物
有机化学
作者
Yanhua Wang,Guiling Yang,Weifeng Shi,Chao Xu,Shanshan Di,Dou Wang,Xinfang Li,Xinquan Wang,Qiang Wang
标识
DOI:10.1016/j.envpol.2020.115067
摘要
Aquatic organisms are usually exposed to various co-existing pollutants. However, toxic effects of pesticide mixtures on aquatic organisms and its potential underlying mechanism still remain unclear. The joint effects of fenpropathrin (FEN) and paclobutrazol (PAC) on zebrafish (Danio rerio) using diverse toxicological endpoints were investigated in the current work. Our data exhibited that the 96-h LC50 values of FEN to zebrafish at multiple life phases ranged from 0.0029 (0.0013–0.0042) to 0.16 (0.082–0.23) mg a.i. L−1, which were lower by comparison to PAC ranging from 13.16 (8.564–21.03) to 23.43 (17.94–29.91) mg a.i. L−1. Combination of FEN and PAC displayed synergistic effect on embryonic zebrafish. Activities of T-SOD, Cu/Zn-SOD and CYP450 were remarkably changed in the majority of single and mixture treatments by comparison to the untreated group. The mRNA levels of 17 genes related to oxidative stress, cellular apoptosis, immune system and endocrine system were assessed, and the data suggested that embryonic zebrafish were affected by both single pesticides and their mixtures. Five genes (P53, tsh, ERα, crh and cxcl-clc) showed greater alterations when exposed to pesticide mixtures by comparison to their individual chemicals. Therefore, it is urgently necessary to conduct more studies on mixture toxicities of different pesticides to explore the chemical mixtures with synergistic interactions.
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