AbstractVariations in the essential oil composition of Matricaria chamomilla L. for a two-year trial were determined. The flowers of Matricaria chamomilla L. were collected from Ankara University gardens in the years of 2011 and 2012. Essential oils were prepared by the hydrodistillation of air-dried plants and analyzed by GC-MS methods. A total of 20 and 16 components were identified, representing over 96.6 % and 93.4 % of the total oil percentage for 2011 and 2012, respectively. The dominant constituents for 2011 were bisabolone oxide (47.6 %), bisabolol oxide A (27.4 %) and en-yn-dicycloether (5.6 %). As for 2012, the principal biologically active compound was bisabolol oxide A (75.4 %), followed by en-yn-dicycloether (4.4 %) and bisabolol oxide B (4.1 %).Key words: Matricaria chamomilla Lessential oilBisabolone oxideBisabolol oxide