咖啡酸
白细胞介素8
奎宁酸
化学
肿瘤坏死因子α
细胞因子
绿原酸
分泌物
趋化因子
白细胞介素
内分泌学
内科学
生物化学
生物
抗氧化剂
医学
食品科学
受体
作者
Zhaohui Zhao,Hee Soon Shin,Hideo Satsu,Mamoru Totsuka,Makoto Shimizu
摘要
Although chlorogenic acid (CHA) easily reaches a millimolar level in the gastrointestinal tract because of its high concentration in coffee and fruits, its effects on intestinal epithelial cells have been little reported. We investigated in this study the down-regulative effects of 5-caffeoylquinic acid (CQA), the predominant isomer of CHA, on the H2O2- or TNF-α-induced secretion of interleukin (IL)-8, a central pro-inflammatory chemokine involved in the pathogenesis of inflammatory bowel diseases, in human intestinal epithelial Caco-2 cells. After the cells had been pre- and simultaneously treated with CQA, the oversecretion of IL-8 and overexpression of its mRNA induced by H2O2 were significantly suppressed in a dose-dependent manner in the range of 0.25–2.00 mmol/L. We further found that a metabolite of CQA, caffeic acid (CA), but not quinic acid, significantly inhibited the H2O2-induced IL-8 secretion and its mRNA expression in the same dose-dependent manner. Both CQA and CA suppressed the TNF-α-induced IL-8 secretion as well. Caffeic acid at 2.00 mmol/l was able to absolutely block the H2O2- or TNF-α-induced oversecretion of IL-8 in Caco-2 cells. However, CQA and CA did not suppress the TNF-α-induced increase in the IL-8 mRNA expression, indicating that the suppressive mechanisms are different between TNF-α-induced and H2O2-induced IL-8 production models. These results suggest that the habit of drinking coffee and/or eating fruits with a high CHA content may be beneficial to humans in preventing the genesis of inflammatory bowel diseases.
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