脂质体
聚乙烯醇
聚合物
化学
体内分布
色谱法
剂型
毒品携带者
磷脂酰胆碱
涂层
药物输送
阿霉素
材料科学
有机化学
膜
生物化学
医学
磷脂
外科
体外
化疗
作者
Hirofumi Takeuchi,Hiroyuki Kojima,Toshitada Toyoda,Hiromitsu Yamamoto,Tomoaki Hino,Yasunaru Kawashima
标识
DOI:10.1016/s0939-6411(99)00029-6
摘要
The purpose of this study was to evaluate the functions of a modified polyvinyl alcohol (PVA-R), which has a hydrophobic moiety, as a coating material for liposomes to be loaded with the anticancer drug, doxorubicin. The size controlled liposomes (egg phosphatidylcholine: cholesterol=1:1 molar ratio) were prepared by the hydration method followed by extrusion. Drug encapsulation and surface modification with polymers (PVA and PVA-R) were carried out simultaneously using a modified pH gradient method. The existence of a thick polymer layer on the surface of the liposomes was confirmed by an increase in particle size and the amount of polymer on the liposomal surface, especially for the PVA-R-coated liposomes. The effects of polymer coating on the behavior of the liposomes in vivo were evaluated by measuring the circulation time and biodistribution of the drug after i.v. administration of the liposomal drug in rats. The PVA-R-coated liposomes showed a more prolonged circulating time for the drug with less uptake by the reticuloendothelial system after i.v. administration in rats, compared with non-coated liposomes. These results confirm that polymer possessing a hydrophobic anchor at its end, like PVA-R, is a suitable material for modifying the surface of doxorubicin-loaded liposomes to improve their stability in the circulating blood.
科研通智能强力驱动
Strongly Powered by AbleSci AI