The culture of Botrytis cinerea and its infection on Arabidopsis.

The optimal condition of gray mold Botrytis cinerea in experiment were potato agar medium plus 1.5 % dextrose at 18-26 ℃ and 8 hours light per day. When inoculated to Columbia,an ecotype of Arabidopsis thaliana,it could be microscopically observed that the pathogen conidia moved over epidermal cell walls,germinated and developed germinating tuber in the first day,then appressoria and invasion pricks formed next day, the hyphae could be seen between mesophyllic cells into third day,abundant hyphae and visible symptoms in Columbia leaves could be observed 4 days after inoculation,and in the sixth or seventh day,the conidiophores were developed and conidia were reproduced.Observation to the macroscopical and microscopical process indicated that the host and pathogen were compatible.