丝兰
生长素
水稻
生物
基因
突变体
生物合成
吲哚-3-乙酸
基因表达
报告基因
细胞生物学
生物化学
植物
作者
Yuko Yamamoto,N. Kamiya,Yoichi Morinaka,Makoto Matsuoka,Takashi Sazuka
出处
期刊:Plant Physiology
[Oxford University Press]
日期:2007-01-12
卷期号:143 (3): 1362-1371
被引量:337
标识
DOI:10.1104/pp.106.091561
摘要
Although indole-3-acetic acid (IAA), the predominant auxin in plants, plays a critical role in various plant growth and developmental processes, its biosynthesis and regulation have not been clearly elucidated. To investigate the molecular mechanisms of IAA synthesis in rice (Oryza sativa), we identified seven YUCCA-like genes (named OsYUCCA1-7) in the rice genome. Plants overexpressing OsYUCCA1 exhibited increased IAA levels and characteristic auxin overproduction phenotypes, whereas plants expressing antisense OsYUCCA1 cDNA displayed defects that are similar to those of rice auxin-insensitive mutants. OsYUCCA1 was expressed in almost all of the organs tested, but its expression was restricted to discrete areas, including the tips of leaves, roots, and vascular tissues, where it overlapped with expression of a beta-glucuronidase reporter gene controlled by the auxin-responsive DR5 promoter. These observations are consistent with an important role for the rice enzyme OsYUCCA1 in IAA biosynthesis via the tryptophan-dependent pathway.
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