Purification and Identification of Lactoferrin from Bovine Milk

Lactoferrin has many kinds of bioactivities which have attracted more and more attention. In the present study, lactoferrin from bovine milk was isolated and purified by membrane filtration, series of chromatography on SP Sepharose Big Bead ion exchange column and Superdex 200 gel filtration column. The purified lactoferrin was identified by SDS-PAGE compared with the lactoferrin standard.