循环肿瘤细胞
液体活检
DNA
癌症研究
转移
生物
计算生物学
生物化学
癌症
遗传学
作者
Runchi Zhang,Siyu Cao,Shiqi Yang,Xiaochen Tang,Peng Sun,Yichun Mao,Guifang Chen,Wenhao Weng,Xiaoli Zhu
标识
DOI:10.1021/acsami.3c15879
摘要
Circulating tumor cells (CTCs) are the "seeds" for malignant tumor metastasis, and they serve as an ideal target for minimally invasive tumor diagnosis. Abnormal glycolysis in tumor cells, characterized by glycometabolism disorder, has been reported as a universal phenomenon observed in various types of tumors. This provides a potential powerful tool for universal CTC capture. However, to the best of our knowledge, no metabolic glycoengineering-based CTC capture strategies have been reported. Here, we proposed a nondestructive CTC capture method based on metabolic glycoengineering and a nanotechnology-based proximity effect, allowing for highly specific, sensitive, and universal CTC capture. To achieve this goal, cells are first labeled with DNA tags through metabolic glycoengineering and then captured through a DNA tetrahedra-functionalized dual-tentacle magnetic nanodevice. Due to the difference in metabolic performance, only tumor cells are labeled with more densely packed DNA tags and captured through enhanced intermolecular interaction mediated by the proximity effect. In summary, we have constructed a versatile platform for nondestructive CTC capture, offering a novel perspective for the application of CTC liquid biopsy in tumor diagnosis and treatment.
科研通智能强力驱动
Strongly Powered by AbleSci AI