甘油三酯
胰岛素抵抗
药理学
胰岛素耐受试验
炎症
医学
肿瘤坏死因子α
胰岛素
胰岛素受体
碳水化合物代谢
糖尿病
内分泌学
内科学
作用机理
胆固醇
生物
生物化学
胰岛素敏感性
体外
作者
Yongjie Wei,Jiaxin Sun,Liya Su,Xu Tian
标识
DOI:10.1177/09731296231215577
摘要
Background The increasing prevalence of type 2 diabetes mellitus (T2DM) on a global scale has created a pressing demand for novel treatments. Gentianella turkestanorum (Gand.) Holub, a traditional Chinese herbal medicine, has been found to possess hypoglycemic effects. However, the mechanism of its action remains unclear. Objective This study was to investigate the impact and mechanism of G. turkestanorum’s water extract (WEG) in reducing insulin resistance (IR) in T2DM. Materials and Methods Db/db mice were administered WEG for 8 weeks, during which their body weight, blood glucose (BG), oral glucose tolerance test, islet tolerance test, fasting insulin, total cholesterol, triglyceride, high-density lipoprotein, and low-density lipoprotein were monitored. Additionally, tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) levels in db/db mice were measured using enzyme-linked immunosorbent assay (ELISA). The study also evaluated the impact of WEG on liver injury through hematoxylin-eosin staining. The expression levels of key proteins and genes in both insulin signaling and inflammation-related pathways were detected using western blotting and real-time quantitative polymerase chain reaction. Results WEG has the potential to regulate glycolipid metabolism, reduce inflammation, and alleviate IR. The mechanism of action may involve promoting the insulin signaling pathway and inhibiting inflammation. Conclusion Gentianella turkestanorum could be a viable treatment option for T2DM and IR.
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