Chromatin buffers torsional stress during transcription

During eukaryotic transcription, Pol II must overcome nucleosome obstacles and, because of DNA’s helical structure, must also rotate relative to DNA, generating torsional stress. However, there is limited understanding of how Pol II transcribes through nucleosomes while supercoiling DNA. Here, we determined that Pol II generates a torque of 9 pN·nm alone and 13 pN·nm with TFIIS, making it a powerful rotary motor. When Pol II encounters a nucleosome, passage becomes more efficient on a chromatin substrate than on a single-nucleosome substrate, demonstrating that chromatin significantly buffers torsional stress during transcription. Furthermore, topoisomerase supercoiling relaxation allows Pol II to transcribe through multiple nucleosomes. Our results reveal a role of chromatin beyond the more conventional view of it being just a roadblock to transcription.