间充质干细胞
转录组
表型
生物
脂肪生成
免疫系统
干细胞
再生医学
体内
多能干细胞
计算生物学
免疫学
癌症研究
细胞生物学
基因表达
遗传学
基因
祖细胞
作者
Hongwei Chen,Xin Wen,Shanshan Liu,Tiffany Sun,Hua Song,Fang Wang,Jiayue Xu,Yueyang Zhang,Yuanjin Zhao,Jia Yu,Lingyun Sun
标识
DOI:10.1002/advs.202202510
摘要
Mixed human umbilical cord-derived mesenchymal stem cells (UC-MSCs) are widely applied in clinical trials to treat various diseases due to their multipotent differentiation potential and immune regulatory activities. However, the lack of a clear understanding of their heterogeneity hampers their application to precisely treat diseases. Moreover, few studies have experimentally authenticated the functions of so-called UC-MSC subpopulations classified from scRNA-seq samples. Here, this work draws a large-scale single-cell transcriptomic atlas and identified three clusters (C1, C2, and C3), representing the primed, intermediate, and stem statuses individually. The C1 and C3 clusters feature higher expression of cytokines and stemness markers, respectively. Surprisingly, further experimental assays reveal that the BAMBIhigh MFGE8high C1 subgroup has a unique phenotype, distinct transcriptomic profile, and limited adipogenic differentiation potential but compromised immunosuppressive activity in vitro and in vivo in lupus mice. Thus, this work is helpful to clarify the nature of human UC-MSCs and to choose optimal MSC types to treat specific diseases in the future.
科研通智能强力驱动
Strongly Powered by AbleSci AI