化学
生物传感器
检出限
对映体
烟酰胺腺嘌呤二核苷酸
乳酸脱氢酶
色谱法
线性范围
试剂
转氨酶
组合化学
酶
生物化学
有机化学
NAD+激酶
作者
Jianling Xu,Meng Wang,Minmin Li,Jinlan Yang,Li Yang
标识
DOI:10.1016/j.aca.2023.341834
摘要
Chiral analysis is of pivotal importance in a variety of fields due to the different biological activities and functions of enantiomers. Here, we develop a simple paper-based chiral biosensor that can perform sample-to-answer simultaneous analysis of lactate enantiomers in human serum samples. By modification of alginate hydrogel with "egg-box" three-dimensional network structure on a glass microfiber paper, reagents of enantiomer-selective enzymatic reactions are efficiently encapsulated forming the sensing regions for chiral analysis. Dual enzyme catalytic system (lactate dehydrogenase and glutamic pyruvic transaminase) is utilized to enhance the response of the biosensor. A smartphone with color analysis software is used to collect and analyze the fluorescence signal from the product nicotinamide adenine dinucleotide. The results show that the sensor has excellent selectivity toward lactate enantiomers with low limit-of-detection of (30.0 ± 0.7) μM for L-lactate and (3.0 ± 0.2) μM for D-lactate, and wide linear detection range of 0.1-3.0mM and 0.01-0.5 mM for L-lactate and D-lactate respectively. The proposed method is successfully applied to the simultaneous detection of L-/D-lactate concentrations in human serum with satisfactory accuracy. Our study provides a robust approach for developing chiral biosensors, which would have promising application prospect in point-of-care testing (POCT) analysis of various biological and food samples.
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