农业渗透
烟草响尾蛇病毒
基因沉默
生物
基因
根癌农杆菌
克隆(编程)
病毒
反向遗传学
遗传学
功能(生物学)
病毒学
计算生物学
基因组
转基因
计算机科学
程序设计语言
作者
Elio Fantini,Giovanni Giuliano
出处
期刊:Methods in molecular biology
日期:2016-01-01
卷期号:: 65-78
被引量:15
标识
DOI:10.1007/978-1-4939-3115-6_7
摘要
Virus-Induced Gene Silencing (VIGS) is an excellent reverse genetic tool for the study of gene function in plants, based on virus infection. In this chapter, we describe a high-throughput approach based on VIGS for the study of tomato fruit biochemistry. It comprises the selection of the sequence for silencing using bioinformatics tools, the cloning of the fragment in the Tobacco Rattle Virus (TRV), and the agroinfiltration of tomato fruits mediated by Agrobacterium tumefaciens.
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