Automatic interfacial tension measurement apparatus based on the drop volume method was developed so as to determine interfacial tension between two liquid phases rapidly and accurately. By this apparatus, for pure water and oil, constant values of equilibrium interfacial tension were obtained. Interfacial tension between aqueous solutions of surfactant and oil, and oil solutions of surfactant and water was measured. In all cases, the equilibrium tension values were determined with high reproducibility and reliability of their absolute values was confirmed. Interfacial tension between two liquid phases could be determined rapidly and automatically with high accuracy, and thus the present apparatus should prove quite using for examining various interfacial phenomena.