Quantitative Determination of Pepsinogen Using the Light Initiated Chemiluminescent Immunoassay

Objective To develop a light initiated chemiluminescent immunoassay for human pepsinogen.Methods Double antibody sandwich immunoassay was used to develop to detect the concentrations of pepsinogen Ⅰ and pepsinogen Ⅱ in human sera respectively.Analytical sensitivity,recovery and within-run precision were evaluated and the results were compared with Abbott reagent(chemiluminescent immunoassay).Results The analytical sensitivity of pepsinogen Ⅰ and pepsinogen Ⅱ were 0.8ng/mL and 0.6ng/mL,recovery of PGⅠ and PGⅡ were 100.3%,106.5%,within-run precision were 0.93%-4.1%、1.2%-4.3%.The coincidence rate with the chemiluminescent immunoassay was highly significant(r=0.99).Conclusions The light initiated chemiluminescent immunoassay for pepsinogen have good sensitivity,precision,accuracy,and can be applied for clinical determination.It will be helpful to carry on the long-term stability test.