Study on determination methods of anthraquinones component of rhubarb

Objective To establish a determination method of anthraquinones of rhubarb.Methods To contrast three extract methods of anthraquinones component of rhubarb,developed a method, anthraquinones were determined using UV spectrophotometry method .Results Anthraqiunones component had a good linearily in the concentration range of 0.856~25.680 μg/ml(r=0.9999).The average recovery was 100.8% with RSD of 0.34%.Conclusion This method is simple,Accurate and reliable,and can be used for the determination of anthraquinones component of rhubarb.