[Objective] The research aimed to select the potential antiobesity ingredients from Portulaca oleracea L..[Method] The proliferation of preadipocytes was detected by methyl thiazolyl tetrazolium(MTT) assay method.[Result] The polysaccharides,alkaloids and flavonoids from Portulaca oleracea L.all displayed good fat inhibiting effects on the proliferation of preadipocyte 3T3-L1,while no significant fat inhibiting effects was seen on unsaturated fatty acid from Portulaca oleracea L.The alkaloids and flavonoids from Portulaca oleracea L.showed higher inhibiting effects at higher concentrations.[Conclusion] The polysaccharides,alkaloids and flavonoids from Portulaca oleracea L.had fat-inhibitting effects.