MnO 2 –Pd/PAAS Hydrogels With OXD‐Like and SOD‐Like Dual Enzymatic Activities Accelerate Infected Wound Healing by Inhibiting Bacterial Growth and Mitigating Oxidative Stress

Abstract Bacterial infections and excessive inflammatory responses are major obstacles to infected wound healing. The MnO 2 –Pd/PAAS hydrogels prepared in this study cound effectively improve the wound microenvironment and promoted wound healing by integrating antibacterial and anti‐inflammatory. The MnO 2 –Pd/PAAS hydrogels prepared via one‐pot self‐assembly method had a high specific surface area due to their flower‐like structure, which enhanced the enzyme‐like catalytic activity. Under acidic conditions, the MnO 2 –Pd/PAAS hydrogels possessed oxidase (OXD) ‐like activity, which cound generate reactive oxygen species (ROS) to kill Methicillin‐resistant Staphylococcus aureus ( MRSA ) and Escherichia coli ( E. coli ). Under neutral conditions, it exhibited superoxide dismutase (SOD)‐like activity with good free radical scavenging activity. Moreover, the MnO 2 –Pd/PAAS hydrogels promoted fibroblast migration and macrophage polarization. In vivo studies demonstrated that the MnO 2 –Pd/PAAS hydrogels cound effectively promote the infected wounds healing with a good biosafety profile. RNA‐seq analysis revealed that MnO 2 –Pd/PAAS hydrogel not only inhibited bacterial growth by inducing ROS production and interfering with ribosome function but also enhanced the immune response by activating T cells and accelerates wound repair by promoting cell migration. In brief, the MnO 2 –Pd/PAAS hydrogels provide an innovative strategy for the treatment of infected wounds.