光合作用
甘油醛3-磷酸脱氢酶
感应(电子)
叶绿体
光系统II
生物
转基因番茄
光系统I
光抑制
转基因
植物
基因表达
转基因作物
化学
生物化学
基因
物理化学
作者
Xiaocui Ma,Chong Chen,Minmin Yang,Xinchun Dong,Wei Lv,Qingwei Meng
标识
DOI:10.1016/j.plaphy.2018.01.003
摘要
Chilling stress severely affects the growth, development and productivity of crops. Chloroplast, a photosynthesis site, is extremely sensitive to chilling stress. In this study, the functions of a gene encoding a cold-regulated protein (SlCOR413IM1) under chilling stress were investigated using sense and antisense transgenic tomatoes. Under chilling stress, SlCOR413IM1 expression was rapidly induced and the sense lines exhibited better growth state of seedlings and grown tomato plants. Overexpression of SlCOR413IM1 alleviated chilling-induced damage to the chloroplast membrane and structure, whereas suppression of SlCOR413IM1 aggravated the damage to chloroplast. Moreover, the net photosynthetic rate (Pn), maximum photochemical efficiency of photosystem II (PSII) (Fv/Fm), actual photochemical efficiency of PSII (ΦPSII) and the activities of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and stromal fructose-1, 6-bisphosphatase (sFBPase) were higher in the sense lines than those in the antisense lines. Hence, the inhibition of photosynthetic capacity was less severe in the sense lines but more severe in the antisense lines compared with that in wild-type (WT) plants. Taken together, overexpression of SlCOR413IM1 enhanced the chilling stress tolerance, whereas suppression of this gene increased the chilling sensitivity of tomato plants.
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