佐剂
抗原
乳酸乳球菌
重组DNA
微生物学
免疫系统
抗体
异源的
生物
细菌
类病毒颗粒
化学
病毒学
免疫学
生物化学
基因
乳酸
遗传学
作者
Maarten L. van Roosmalen,Rolf Kanninga,Mohamed El Khattabi,Jolanda Neef,Sandrine A. L. Audouy,Tjibbe Bosma,Anneke Kuipers,Eduard Post,Anton Steen,Jan Kok,Girbe Buist,Oscar P. Kuipers,George T. Robillard,Kees Leenhouts
出处
期刊:Methods
[Elsevier]
日期:2006-02-01
卷期号:38 (2): 144-149
被引量:93
标识
DOI:10.1016/j.ymeth.2005.09.015
摘要
Mucosal immunization with subunit vaccines requires new types of antigen delivery vehicles and adjuvants for optimal immune responses. We have developed a non-living and non-genetically modified gram-positive bacterial delivery particle (GEM) that has built-in adjuvant activity and a high loading capacity for externally added heterologous antigens that are fused to a high affinity binding domain. This binding domain, the protein anchor (PA), is derived from the Lactococcus lactis AcmA cell-wall hydrolase, and contains three repeats of a LysM-type cell-wall binding motif. Antigens are produced as antigen-PA fusions by recombinant expression systems that secrete the hybrid proteins into the culture growth medium. GEM particles are then used as affinity beads to isolate the antigen-PA fusions from the complex growth media in a one step procedure after removal of the recombinant producer cells. This procedure is also highly suitable for making multivalent vaccines. The resulting vaccines are stable at room temperature, lack recombinant DNA, and mimic pathogens by their bacterial size, surface display of antigens and adjuvant activity of the bacterial components in the GEM particles. The GEM-based vaccines do not require additional adjuvant for eliciting high levels of specific antibodies in mucosal and systemic compartments.
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