Purification of Astragalus Polysaccharide with Macroporous Resins

The adsorption and desorption properties of Astragalus Polysaccharide (APS) (70% content) on Macroporous resins including X-5, AB-8 and D101 have been compared. APS solutions of different concentrations were tested to find a suitable sample concentration on the macroporous resins. According to our test results, AB-8 performs better than other resins. The adsorption capacity and desorption rate are 41.73mg/ml and 83.74%, higher adsorption speed for APS. Column chromatograph with AB-8 resin was used for the purification test, the sample concentration of APS is 20mg/ml, the purity of APS can reach 94.68%.