共核细胞病
诱导多能干细胞
神经科学
生物
α-突触核蛋白
人脑
生物神经网络
帕金森病
体外
细胞生物学
疾病
胚胎干细胞
基因
遗传学
医学
病理
作者
Simona Gribaudo,Luc Bousset,Josquin Courte,Ronald Melki,Jean‐Michel Peyrin,Anselme L. Perrier
出处
期刊:Methods in molecular biology
日期:2022-10-31
卷期号:: 357-378
标识
DOI:10.1007/978-1-0716-2597-2_24
摘要
Aggregated alpha-synuclein (α-Syn) in neurons is a hallmark of Parkinson's disease (PD) and other synucleinopathies. Recent advances (1) in the production and purification of synthetic assemblies of α-Syn, (2) in the design and production of microfluidic devices allowing the construction of oriented and compartmentalized neuronal network on a chip, and (3) in the differentiation of human pluripotent stem cells (hPSCs) into specific neuronal subtypes now allow the study of cellular and molecular determinants of the prion-like properties of α-Syn in vitro. Here, we described the methods we used to reconstruct a cortico-cortical human neuronal network in microfluidic devices and how to take advantage of this cellular model to characterize (1) the prion-like properties of different α-Syn strains and (2) the neuronal dysfunctions and the alterations associated with the exposure to α-Syn strains or the nucleation of endogenous α-Syn protein in vitro.
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