Genome-wide identification of FAR gene family and functional analysis of NlFAR10 during embryogenesis in the brown planthopper Nilaparvata lugens

Fatty acyl-CoA reductases (FARs) catalyze the synthesis of fatty alcohols from corresponding fatty acid precursors in organisms. However, the function of FARs in insect fecundity and embryogenesis remains largely unclear. Here, a total of 22 putative FAR proteins were identified in the brown planthopper Nilaparvata lugens, a hemipteran insect pest of rice, and most of them were highly expressed in embryonic stages. Among them, NlFAR10 was specifically and highly expressed in the later embryogenesis, but was promiscuously expressed in tissues of adults. The heterologously expressed NlFAR10 was able to produce the intermediate fatty acid alcohols from the corresponding acyl-CoA precursors. When NlFAR10 was silenced through RNAi in vivo, the embryogenesis was obviously inhibited, resulting in low hatching rates. Moreover, the metabolome analyses indicated that loss of NlFAR10 affected lipid metabolism and purine metabolism during embryogenesis. To the best of our knowledge, this is the first report of a FAR member affecting insect embryogenesis, thus providing a new target for future pest management.