烧伤
总体表面积
真皮
烧伤
热烧伤
医学
败血症
免疫系统
表皮(动物学)
凝固性坏死
大鼠模型
炎症
伤口愈合
皮肤病科
病理
免疫学
外科
内科学
解剖
作者
Rajnikant Sharma,Shekhar Yeshwante,Quentin Vallé,Maytham Hussein,Varsha J. Thombare,Sean McCann,Robert Maile,Jian Li,Tony Velkov,Gauri G. Rao
摘要
Burn induction methodologies are inconsistently described in rat models. A uniform burn wound model, which represents the clinical scenario, is necessary to perform reproducible burn research. The present protocol describes a simple and reproducible method to create ~20% total body surface area (TBSA) full-thickness burns in rats. Here, a 22.89 cm2 (5.4 cm diameter) copper rod heated at 97 °C in a water bath was applied to the rat skin surface to induce the burn injury. A copper rod with a high thermal conductivity was able to dissipate the heat deeper in the skin tissue to create a full-thickness burn. Histology analysis shows attenuated epidermis with coagulative damage to the full-thickness extent of the dermis and the subcutaneous tissue. Additionally, this model is representative of the clinical situations observed in hospitalized burn patients following burn injury such as immune dysregulation and bacterial infections. The model can recapitulate the systemic bacterial infection by both Gram-positive and Gram-negative bacteria. In conclusion, this paper presents an easy-to-learn and robust rat burn model that mimics the clinical situations, including immune dysregulation and bacterial infections, which is of considerable utility for the development of new topical antibiotic drugs for burn wound and infections.
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