精子细胞
精子发生
生物
基因敲除
精子
生殖系
果蝇属(亚属)
细胞生物学
Piwi相互作用RNA
遗传学
不育
黑腹果蝇
RNA干扰
核糖核酸
基因
内分泌学
作者
Di Xu,Jing Pan,Fang Yang,Long Zhao,Ying Su
标识
DOI:10.1016/j.bbrc.2024.149633
摘要
Ribosomal protein 25 (RPS25) has been related to male fertility diseases in humans. However, the role of RPS25 in spermatogenesis has yet to be well understood. RpS25 is evolutionarily highly conserved from flies to humans through sequence alignment and phylogenetic tree construction. In this study, we found that RpS25 plays a critical role in Drosophila spermatogenesis and its knockdown leads to male sterility. Examination of each stage of spermatogenesis from RpS25-knockdown flies showed that RpS25 was not required for initial germline cell divisions, but was required for spermatid elongation and individualization. In RpS25-knockdown testes, the average length of cyst elongation was shortened, the spermatid nuclei bundling was disrupted, and the assembly of individualization complex from actin cones failed, resulting in the failure of mature sperm production. Our data revealed an essential role of RpS25 during Drosophila spermatogenesis through regulating spermatid elongation and individualization.
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