Light-driven cofactor-free hydroxylation driven by P450 BM3@g-C3N4

Activation of C-H is of great significance in the construction of complex molecular scaffolds in biomedical field. In recent years, enzyme catalysis has received widespread attention due to the high regio- and stereoselectivity and conforms to the current low-carbon policy, more and more reactions realize the non-natural C-H activation by reforming P450s. However, addition of NADPH cofactor will increase costs and may cause side effects. In order to circumvent the dependency on the reductase, we utilized biocompatible g-C₃N₄ porous nanosheets modified with P450 BM3 to form a simple, green and low-cost enzyme-photocoupled biocatalytic system for efficient solar-driven C–H oxidation. Finally, 25 μmol/L 11- hydroxy laurate was produced by photocatalysis of lauric acid, P450 BM3@C₃N₄ and TEOA for 3 h.