Exploring the hypoglycaemic efficacy of bio-accessed antioxidative polyphenolics in thermally processed Cucumis dipsaceus fruits – An in vitro and in silico study

Inhibition of breakdown of dietary carbohydrates, by controlling the postprandial activity of diabetic enzymes through fruit polyphenolics can help downregulate the effects of Type 2 Diabetes Mellitus (T2DM). The study focuses on deciphering the induction of hyperglycaemic control by bio-accessed anti-oxidative polyphenols of Cucumic dipsaceus fruits. Chiefly, we examined the antioxidant activity of bio-accessed polyphenols of C. dipsaceus fruits (DPPH: ME (GDE)-66.26 %; ABTS: FE (IDE)-1963.83 µM TEAC/mg extract; Phosphomolybdenum reduction: FE (IDE)- 64.95 mg AAEAC/g extract). To add more significance, the anti-diabetic activity was predetermined by in silico docking analyses (Pseudojervine - -5.43; Squalene- -10.41) and was concurrently confirmed by in vitro studies (α amylase inhibition: ME (GDE) - 69.58 %; α glucosidase inhibition: FE (UDE)- 88.67 %). A higher bio-accessibility of rutin (37.92 mg/g ODE) and gallic acid (8.36 mg/g ODE) was observed after quantification by HPLC, which confirmed the correlation between anti-diabetic activity and C. dipsaceus fruit phenolic compounds.