Engineering Efficient Biosynthesis of Baicalein-7- O -xyloside in Escherichia coli via Glycosyltransferase Evolution and Metabolic Pathway Optimization

achieved efficient biotransformation. With metabolic and fermentation optimization, the titer reached 198.4 ± 3.9 mg/L. This study provides an efficient, selective route to baicalein xyloside by combining enzyme and metabolic engineering, enabling scalable production of bioactive glycosides.