A red emission multiple detection site probe for detecting carboxylesterase 1 based on BODIPY fluorophore

In this work, a BODIPY-based multi-site red emission fluorescent probe MRS-BOD for the detection of carboxylesterase 1 (CES1) was designed and synthesized. MRS-BOD showed good biocompatibility and could be used to monitor the activity of CES1 in living cells. MRS-BOD emission is located in the red emission region, which has the advantages of less background interference and less damage to biological samples. MRS-BOD has multiple CES1 recognition sites, and the ultra-sensitive quantification of CES1 has been successfully achieved. The low detection limit of MRS-BOD was calculated as 2.5 ng/mL, and the fluorescence quantum yield was calculated as 0.49. This study can promote the development of CES1 fluorescent probes and help the identification of related diseases.